منابع مشابه
RNA modifications go viral
Viral life cycles are often coordinated by precise mechanisms that act on their RNA. For example, the microRNA miR-122 interacts with the viral RNA genome of hepatitis C virus (HCV) and is required for HCV replication [1]. In the past year, several groups have reported a new RNA regulatory control to viral infection—the posttranscriptional RNA modification N6methyladenosine (mA). This reversibl...
متن کاملQuantitative analysis of RNA modifications.
RNA modifications impact numerous cellular processes such as pre-mRNA splicing and protein synthesis. The elucidation of the mechanisms by which these modifications impact cellular processes necessitates the ability to both detect and quantify the presence of these modifications within RNA molecules. Here, we present a detailed procedure that allows the detection and quantification of RNA base ...
متن کاملDetection and quantitation of RNA base modifications.
Using a new combination of previously published techniques, we developed a method for quantitating modified nucleotides in RNAs. First, an RNA is cleaved with RNase H at the 5' side of a nucleotide of interest. Next, 32P is substituted for the phosphate at the 5' end of this nucleotide. Finally, after nuclease P1 digestion, the released radiolabeled nucleotide is analyzed by thin layer chromato...
متن کاملNew Edges of RNA Adenosine Methylation Modifications
Recently an article published in Molecular Cell reveals the mechanism of a nuclear N-methyladenosine (mA) reader, the YTH domain-containing protein 1 (YTHDC1), in regulating pre-mRNA splicing [1]. Meanwhile, two additional articles published in Nature and Nature Chemical Biology report the first transcriptome-wide maps of N-methyladenosine (mA) at high resolution, suggesting a functional role f...
متن کاملRecent Advances in Identification of RNA Modifications
RNA modifications are involved in a broad spectrum of biological and physiological processes. To reveal the functions of RNA modifications, it is important to accurately predict their positions. Although high-throughput experimental techniques have been proposed, they are cost-ineffective. As good complements of experiments, many computational methods have been proposed to predict RNA modificat...
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ژورنال
عنوان ژورنال: Nature
سال: 2017
ISSN: 0028-0836,1476-4687
DOI: 10.1038/nature24156